10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 3 2012 (July - September) lessThan i greaterThan Ecbolium linneanum lessThan /i greaterThan is used conventionally to treat various diseases like jaundice, rheumatism and also used as anti-inflammatory agents. Pharmacological properties of the leaf and stem of lessThan i greaterThan E. linneanum lessThan /i greaterThan were evaluated by antioxidant potential and cytotoxic activity. Ethanol, acetone, dichloromethane and petroleum ether extracts of leaf and stem of lessThan i greaterThan E. linneanum lessThan /i greaterThan were prepared to study the total phenolic content, radical scavenging activity, reducing power assay, total antioxidant capacity and cytotoxic by multiple lessThan i greaterThan in vitro lessThan /i greaterThan assays. The total phenolic content, analysed by using Folin- Ciocalteau reagent, varied from 33.3 to 129.00 mg/g dry weight. The results showed that leaf and stem extracts of lessThan i greaterThan E. linneanum lessThan /i greaterThan possesses antioxidant and free radical scavenging properties. Moreover, cytotoxicity was also examined lessThan i greaterThan in vitro lessThan /i greaterThan in HeLa cervical cancer cell line by trypan blue assay and ethanolic extract of leaf exhibited over 80% cell death. This study revealed that the activities observed could be attributed to the presence of some phytochemicals which have been associated with antioxidant and cytotoxic property. Dipankar C And Murugan S Ecbolium linneanum, antioxidant, cytotoxicity, DPPH, HeLa 112-120