10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 2 2012 (April - June) A survey of isozyme polymorphism among glucose and non-glucose metabolizing enzymes was carried out in the laboratory population of lessThan i greaterThan Sarcophaga dux lessThan /i greaterThan . Polymorphic levels were found to be within the range recorded for other dipterans. Polymorphism among glucose-metabolizing enzymes were found to be on an average less variable (H lessThan sub greaterThan o lessThan /sub greaterThan =0.186; H lessThan sub greaterThan e lessThan /sub greaterThan =0.163) than the non-glucose metabolizing enzymes (H lessThan sub greaterThan o lessThan /sub greaterThan =0.275; H lessThan sub greaterThan e lessThan /sub greaterThan =0.485). Out of five loci, three loci (LDH-1, ACPH-1 and APH-1) were found to be polymorphic and these displayed significant deficiency of heterozygotes. These results supported the earlier findings that the substrate-specific enzymes with functional constraints have lower heterozygosity than the non-specific enzymes. They also added to the evidence obtained from other animals like lessThan i greaterThan Drosophila lessThan /i greaterThan , echinoderms and some fish species. Kiran Singh And Sasya Thakur isozyme polymorphism, glucose metabolizing enzymes, non-glucose metabolizing enzymes, functional constraint, neutral mutation, Sarcophaga dux 638-644