International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 3 Issue 2
2012 (April - June)
Green Synthesis Of Silver Nanoparticles Using Leaves Of Lepisanthes Tetraphylla And Evaluation Of Their Antibacterial Activity Against Drug Resistant Clinical Isolates
The silver nanoparticles (AgNPs) synthesized using aqueous leaf extract of lessThan i greaterThan Lepisanthes tetraphylla lessThan /i greaterThan as the reducing agent were evaluated for antibacterial activity against drug resistant bacterial isolates. The nature of AgNPs synthesized were analysed by UV-Vis spectroscopy, X-ray diffraction and Scanning electron microscopy. The silver nanoparticles were with an average size of 30nm, spherical and poly-dispersed. The antibacterial potential of synthesized AgNPs were compared with that of crude methanolic leaf extract of Lepisanthes tetraphylla by well diffusion method. The AgNPs at 30-50µg(0.03-0.05mg) concentration significantly inhibited bacterial growth against multi drug resistant lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan , lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Pseudomonas aeruginosa and Acinetobacter species lessThan /i greaterThan . The study revealed that crude methanolic leaf extract of L.tetraphylla inhibited the growth of only lessThan i greaterThan Escherichia coli lessThan /i greaterThan , at 1mg/ml concentration. Thus AgNPs showed broad spectrum antibacterial activity at lower concentration may be a good alternative therapeutic approach in future.
N.Meena, M.Jeya, S.Aroumugame, P.Arumugam And E.Sagadevan
Lepisanthes tetraphylla, antibacterial activity, silver nanoparticles, well diffusion method
593-601