10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 2 2012 (April - June) We report the plantlet regeneration of ICPL 87119 (Asha) Pigeon pea varieties from mature zygotic embryos cultured lessThan i greaterThan in vitro. lessThan /i greaterThan Mature zygotic embryos were cultured on Murashige and Skoog's medium augmented with different concentrations of TDZ (1.0 – 6.0 mg/L) Mature Zygotic embryos were germinated and more number of multiple shoot buds are induced directly at 4.0 mg/L followed by 3.0 mg/L and 2.0mg/L TDZ. These multiple shoots were further sub-cultured on the same fresh medium containing 1.0 mg/L TDZ for further elongation. The micro-shoots were transferred onto rooting medium containing MS+0.5-3.0 mg/L IBA and 0.5–3.0 mg/L IAA alone. The micro-shoots were rooted (100%) on MS-medium fortified with 3.0 mg/L IBA. The regenerated plantlets were hardened in Plant Growth Chamber under controlled conditions (humidity 80%; temp.25 lessThan sup greaterThan 0 lessThan /sup greaterThan C). After 3 weeks, plants were transferred to field. The highest percentage of plantlets (95%) was survived under field conditions and they were found to be morphologically identical to the parental plants. Thus lessThan i greaterThan in vitro lessThan /i greaterThan mature zygotic embryo culture plays a role in the production of plantlets that can be transferred to natural field. G.P.V Shekhar M.Venkateshwarlu T. Srilatha K Jagan Mohan Reddy And T.Ugandhar Mature zygotic embryos, Thiadizuron (TDZ), multiple shoots, In Vitro rooting 300-305