International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 3 Issue 2
2012 (April - June)
Histopathological And Biochemical Studies Of Etoposide Treated Liver Of Rat
Etoposide is a known anticancer drug used for treatment of various tumors. Studies were performed on rats treated with etoposide for 8 weeks. Histopathological alterations like parenchymal ruptures and vacuolation of hepatocyte were observed. The blood sinusoids were dilated and the central blood vessels were also congested with amorphous material. Transmission electron microscopic (TEM) observations showed vesicuolation of smooth and rough endoplasmic, electron dense mitochondria with intact matrix and presence of active golgi apparatus with its characteristic secretory granules and lipid vacuoles. Biochemical findings indicated a significant change in glutathione-s-transferase (G-ST), glutathione reductase (GR), catalase, cytochrome P450 (CYP lessThan sup greaterThan 450 lessThan /sup greaterThan ) and cytochrome b5 (CYP b lessThan sub greaterThan 5 lessThan /sub greaterThan ). However glutathione (GSH), glutathione peroxidase (GPx) and gamma glutamyl transferase (GGT) showed non-significant change. No significant increase was manifested in lipid peroxidation (Lpx) levels in the liver. The metabolic activation of drug by CYP lessThan sup greaterThan 450 lessThan /sup greaterThan displayed significant increase and significant decrease of CYP b lessThan sub greaterThan 5 lessThan /sub greaterThan .These changes demonstrate that etoposide at given dose level is not involved in exerting cytotoxic action on the liver.
Pratibha Ravindra, Sameer R. Kulkarni, C. Y. Dhume And D. A. Bhiwgade
Etoposide, Mitochondria, Endoplasmic Reticulum, Glutathione, Lipid peroxidation.
221-229