International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 3 Issue 2
2012 (April - June)
Isolation, Identification Of Bromophenol Compound And Antibacterial Activity Of Kappaphycus SP
Extraction, isolation and partial purification of bromophenol compound in lessThan i greaterThan Kappaphycus sp lessThan /i greaterThan . was carried out using TLC method. Antibacterial studies for specific bromophenol compound by disc diffusion method were conducted. Identification of a purified component was carried out by NMR technique. FTIRS and mass spectroscopy for elucidation of molecular structure of specific bromophenolic compound were done. From TLC studies, various bands were observed under UV light possessing the maximum value of RF 0.83 corresponding to RF1 band. The band was taken out and tested for antibacterial activity and detection of specific compound. Antimicrobial studies were carried out against various pathogens, lessThan i greaterThan Pseudomonas fluorescence, Staphylococcus aerus lessThan /i greaterThan , lessThan i greaterThan Vibrio cholera lessThan /i greaterThan , lessThan i greaterThan Proteus mirabilis lessThan /i greaterThan and it was observed that the zone of inhibition for specific compound isolated from TLC was very prominent in case of lessThan i greaterThan Pseudomonas fluorescence and Staphylococcus aerus. lessThan /i greaterThan C lessThan sup greaterThan 13 lessThan /sup greaterThan –NMR spectrum of Bromophenol derivative in CdCl lessThan sub greaterThan 3 lessThan /sub greaterThan showed eight Carbon signals. In mass spectrum analysis of Bromophenol compound, the compound was found to contain two Br atoms from the molecular ion peak at m/z 349.1676 and was analyzed for chemical formula C lessThan sub greaterThan 11 lessThan /sub greaterThan H lessThan sub greaterThan 9 lessThan /sub greaterThan O lessThan sub greaterThan 3 lessThan /sub greaterThan Br lessThan sub greaterThan 2 lessThan /sub greaterThan . Based on the above characterization the compound was confirmed as-(E)-3-(2, 3-dibromo 4, 5-dihydroxyphenyl)-2-methyl-propenal.
P. Rajasulochana, P. Krishnamoorthy And R. Dhamotharan
Red algae; Kappaphycus, bromophenol, antibacterial
173-186