10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 2 2012 (April - June) lessThan i greaterThan Gymnema sylvestre lessThan /i greaterThan R. Br lessThan i greaterThan . lessThan /i greaterThan a very useful medicinal plant belongs to the family Asclepiadaceae. Nodal explants of lessThan i greaterThan Gymnema sylvestre lessThan /i greaterThan were used for lessThan i greaterThan in vitro lessThan /i greaterThan multiplication on a medium containing various concentrations of growth regulators such as BA and NAA. Among the combinations used, MS medium fortified with BA at 1.0 mg/l was found to be suitable for induction of multiple shoots. The shoots were observed within 1 week of inoculation. The regenerated shoots were transferred to rooting media supplemented with different concentrations of NAA. The highest percentage of rooting was observed with NAA at 1.0 mg/l. The regenerated plantlets were transferred to the field after acclimatization lessThan b greaterThan . lessThan /b greaterThan R. Manonmani And P. Francisca Gymnema sylvestre R.Br., nodal explants, In vitro multiplication, MS medium. 49-53