10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 2 2012 (April - June) The objective of this investigation was to develop a new auxotropic mutant from a regulatory mutant and to examine its potency for L-lysine production. A high L-lysine yielding strain lessThan i greaterThan Micrococcus glutamicus lessThan /i greaterThan AB lessThan sub greaterThan 200 lessThan /sub greaterThan was developed from a regulatory mutant lessThan i greaterThan Micrococcus glutamicus lessThan /i greaterThan AB lessThan sub greaterThan 5 lessThan /sub greaterThan by subsequent mutagenic treatment with UV rays and methyl methane sulfonate respectively. Treatment of UV rays on the regulatory mutant AB lessThan sub greaterThan 5 lessThan /sub greaterThan resulted in a mutant strain AB lessThan sub greaterThan 50 lessThan /sub greaterThan gives the maximum yield of L-lysine (5.5mg/mL) than the regulatory mutant (0.9 mg/mL). The mutant strain AB lessThan sub greaterThan 50 lessThan /sub greaterThan on further treatment with a chemical mutagen methyl methane sulfonate resulted to another mutant lessThan i greaterThan Micrococcus glutamicus lessThan /i greaterThan AB lessThan sub greaterThan 200 lessThan /sub greaterThan which produced the highest yield of L-lysine (7.2mg/mL) lessThan i greaterThan Micrococcus lessThan /i greaterThan lessThan i greaterThan glutamicus lessThan /i greaterThan AB lessThan sub greaterThan 200 lessThan /sub greaterThan was an auxotropic mutant which requires biotin for growth as well as production of L-lysine. The L-lysine producing capacity of auxotropic mutant was tested after maintaining the strain in three different media of which the mutant was stable in a media having the composition of 5% glucose, 0.6% urea, 0.2% K lessThan sub greaterThan 2 lessThan /sub greaterThan HPO lessThan sub greaterThan 4 lessThan /sub greaterThan . 0.025% MgSO lessThan sub greaterThan 4 lessThan /sub greaterThan . 7H lessThan sub greaterThan 2 lessThan /sub greaterThan O, 2µg/mL biotin. A. Gupta, S. K. Das And A. K. Banik Micrococcus glutamicus AB200, Micrococcus glutamicus AB50,Yeast, Biotin, L-lysine, Auxotroph, UV rays, MMS. 35-42