10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) The Indian spices are known for their medicinal properties from ages apart from adding taste to the Indian cuisines. In this study, traditional Indian spices such as lessThan i greaterThan Cuminum cyminum lessThan /i greaterThan , lessThan i greaterThan Cinnamomum zeylanicu lessThan /i greaterThan , lessThan i greaterThan Eletteria cardamom lessThan /i greaterThan , lessThan i greaterThan Eugenia caryophyllus lessThan /i greaterThan and lessThan i greaterThan Piper nigrum lessThan /i greaterThan were assessed for inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) enzymes. The assessment of cholinesterase enzyme inhibition was carried out by using a colorimetric method based on Ellman's reaction. Our findings demonstrate that aqueous and ethanolic extract of these plants significantly inhibited AChE and BuChE enzymes. For aqueous extracts the maximum inhibitory activity was shown by lessThan i greaterThan Cuminum cyminum lessThan /i greaterThan (66.66±0.005) % and (66.84±0.001) % for AChE and BuChE respectively. For ethanolic extracts, maximum inhibitory activity for AChE was observed for lessThan i greaterThan Eletteria cardamom lessThan /i greaterThan i.e. 61.96±0.003% whereas for BuChE inhibition, lessThan i greaterThan Piper nigrum lessThan /i greaterThan showed maximum inhibition i.e 73.26±0.005%. These results showed that there could be great potential to search for novel usage of these medicinal plants for anti-cholinesterase compounds. S. Kumar, Brijeshlata And S.Dixit Acetylcholinesterase, butyrylcholinesterase, inhibition, Ellman’s method, Alzheimer’s disease 59-65