10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) Electrophoretic profile of blood serum proteins of air breathing fresh water fish lessThan i greaterThan Channa gachua lessThan /i greaterThan was examined. Total eleven protein bands with varying migration on 10% polyacrylamide gel were observed in native run. The slow migrating bands designated as lessThan i greaterThan Cg lessThan /i greaterThan -1, has molecular weights 154.20 Da and moderate migrating bands i.e. lessThan i greaterThan Cg lessThan /i greaterThan -2, lessThan i greaterThan Cg lessThan /i greaterThan -3, lessThan i greaterThan Cg lessThan /i greaterThan -4, lessThan i greaterThan Cg lessThan /i greaterThan -5, lessThan i greaterThan Cg lessThan /i greaterThan -6, lessThan i greaterThan Cg lessThan /i greaterThan -7, lessThan i greaterThan Cg lessThan /i greaterThan -8, lessThan i greaterThan Cg lessThan /i greaterThan -9 have molecular weights 71.61, 63.13, 58.61 56.70, 54.09, 45.29, 42.24 and 35.62 Da respectively. Remaining two fast migrating bands i.e. lessThan i greaterThan Cg lessThan /i greaterThan -10 and lessThan i greaterThan Cg lessThan /i greaterThan -11 have low molecular weights i.e. 16.15 and 14.79 KDa respectively. This protein profile of serum did not alter in the presence of reducing agent i.e. 2-mercaptoethanol on 12% sodium dodyacylsulphate polyacrylamide gel (SDS-PAGE) suggesting that, these proteins consists of single polypeptide chains. M. V.Gaikwad, P.M. Davane ,V.R.More, Shaikh F.K.,Jaiwal B.V., AndY.K. Khillare Protein profile, serum, Channa gachua. Native-PAGE and SDS-PAGE 628-633