10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) Salinity, is one of the most serious environmental factors limiting the productivity of crop plants and agricultural production world-wide. An attempt was made to develop salinity tolerance in Groundnut- lessThan i greaterThan Arachis hypogea lessThan /i greaterThan l. var GG20 through lessThan i greaterThan Agrobacterium lessThan /i greaterThan mediated transformation with the lessThan i greaterThan cox lessThan /i greaterThan gene for synthesis of Glycine betaine. lessThan i greaterThan Agrobacterium tumefaciens lessThan /i greaterThan LBA 4404 with binary vector pHS724 containing the cox gene was co-cultivated with different groundnut explants for infection and transformation. Various factors like explant types and co-cultivation period affecting transformation and concentration of kanamycine in selection medium were studied. The successful transformants were confirmed through phenotypic expression as well as through the expression of GUS gene lessThan i greaterThan (uidA lessThan /i greaterThan gene) in the regenerated plants. Ashutosh Vadawale, Ritu Mihani, Asha Mathew And Pushpa Robin Salinity, Glycine betaine, Groundnut, Transformation 591-599