10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) Electrophoretically detectable genetic variation of five enzyme systems was analyzed in laboratory colonies of blow fly lessThan i greaterThan Chrysomya megacephala lessThan /i greaterThan using Polyacrylamide gel electrophoresis (PAGE). A sample of 50 individuals was analyzed for ten allozymic loci (LDH-1, APH-1, APH-2, APH-3, ACPH-1, ACPH-2, ME-1, ME-2, XDH-1 and XDH-2). Four of them were polymorphic (LDH-1, APH-2, APH-3, and ACPH-2). Among them the LDH-1 locus did not show statistically significant deviation from the Hardy-Weinberg equilibrium, whereas, the remaining polymorphic loci APH-2, APH-3 and ACPH-2 presented deficiency of heterozygotes, possibly due to inbreeding. The estimates of genetic variation with respect to mean heterozygosity, polymorphism and mean number of alleles per locus were found to be in the range described for carrion-breeding as well as non carrion-breeding Dipterans. To the best of our knowledge, this is the first estimate of genetic variation based on allozyme studies in the blow fly lessThan i greaterThan C.megacephala lessThan /i greaterThan . Kiran Singh, Sasya Thakur And P.W.Ramteke Chrysomya megacephala, Calliphoridae, Blowflies, population genetics, allozyme electrophoresis, carrion-breeding dipterans. 538-546