10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) Antimicrobial activity of leaf extract of lessThan i greaterThan Ocimum gratissimum lessThan /i greaterThan L was studied using different solvent like chloroform, acetone, ethanol and water against bacterial strains like lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan , lessThan i greaterThan Staphylococcus aureus, Escherichia coli ,Pseudomonus aeruginosa lessThan /i greaterThan and fungal strains lessThan i greaterThan Aspergillus niger lessThan /i greaterThan and lessThan i greaterThan Penicillum chrysogenum lessThan /i greaterThan . The antimicrobial activity was determined by disc diffusion method. Out of the four extract used, chloroform and acetone extracts were found to be highly active against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Pseudomonas aeruginosa lessThan /i greaterThan Hosamani.P.A, Lakshman.H.C , Sandeepkumar.K And Shyamkumar.V Ocimum gratissimum, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonus aeruginosa, Aspergillus niger and Penicillum chrysogenum 467-472