10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) Wild species of lessThan i greaterThan Bacillus spore formers lessThan /i greaterThan were selectively isolated from various sources like Dry spices, Milk, Soil, Intestine of pig and Chicken .Probiotic potential of isolates were studied on the basis of non hemolysis, non lecithinase activity ,acid bile tolerance, resistance to artificial gastric and intestinal fluids and antagonistic action towards enteric pathogens such as lessThan i greaterThan Salmonella typhi, Salmonella paratyphi A lessThan /i greaterThan ,and lessThan i greaterThan Vibrio cholera lessThan /i greaterThan . Based on probiotic qualities five strains were further selected and their antagonistic principles and susceptibility to commonly used antibiotics were studied.Among the five isolates lessThan i greaterThan Bacillus spp lessThan /i greaterThan BM-3 from milk showed remarkable probiotic and antagonistic properties. Antimicrobial compound produced by BM-3 was heat labile and was not affected by the proteases. This isolate was susceptible to commonly prescribed antibiotics in conflict that they would not donate resistance. Biochemical identification reveals that the potential probiotic isolate lessThan i greaterThan bacillus spp lessThan /i greaterThan BM-3 from milk is lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan and it can be studied lessThan i greaterThan In vivo lessThan /i greaterThan for future development of potential probiotic Anu P Sebastian And T R Keerthi Bacilli, Sporeformers, Probiotic, Enteric pathogens 327-335