10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from cotyledon explants was achieved in Pigeon pea ( lessThan i greaterThan Cajanus cajan lessThan /i greaterThan (L.) cv ICPL 87119 (Asha). Somatic embryos were induced directly from cotyledon explants on Murashige and Skoog's (MS) medium fortified with different concentrations of 2,4- dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, (NAA) Naphthaleneacetic acid, fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supple mented with 2.0 mg/L 2,4-D, 3.0 mg/L 2,4-5-T and 2.0 mg/L NAA, respectively. Embryo development and well maturation was achieved on MS medium supplemented with 2.0mg/L2,4-D + 1.5mg/L TDZ, 3.0mg/L2,4-D + 2.0 mg/L BAP. The well formed embryos germinated into complete plantlets on MS medium containing 2.0 mg/L TDZ and 2.0mg/L NAA (α-Naphthalene acetic acid). The regenerated plants were first transferred to plastic cups and later into pots for maturation, then transferred to soil and grown to maturity with 80%survival and the tissue culture raised plants produced viable seeds. Thirunahari. Ugandhar,M.Venkateshwarlu G.P.V Shekhar, And. K Jagan MohanReddy Cajanus cajan, grain legume, cotyledon, somatic embryogenesis. Abbreviations: 2,4-dichlorophenoxyacetic acid, (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) , Naphthaleneacetic acid (NAA), Thiadizuron (TDZ) and BAP (Benzyl amino purine) 291-298