10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) lessThan i greaterThan In vitro lessThan /i greaterThan antioxidant activities of methanolic extract of tuberous root of lessThan i greaterThan coleous vettiveroides lessThan /i greaterThan (jacob.) was investigated. The free radical scavenging activity to evaluate by DPPH (2,2 -diphenyl -1- picryl hydrazyl) method, Nitric oxide method, Total antioxidant activity. DPPH radical scavenging activity of Methanolic extract and reference standard Rutin IC lessThan sub greaterThan 50 lessThan /sub greaterThan values was found to be 250 mg/ml and 800 mg/ml, nitric oxide scavenging activity of methanolic extract and reference standard ascorpate IC lessThan sub greaterThan 50 lessThan /sub greaterThan values was found to be 510 mg/ml and 490 mg/ml and Total antioxidant activity of methanolic extract was evaluated by phosphomolybdic acid method and reference standard ascorbate IC lessThan sub greaterThan 50 lessThan /sub greaterThan values was found to be 560mg/ml and 585 mg/ml respectively. The above result of possess good antioxidant activity when compared to the above all standards. G.Gopalakrishnan, C.K.Dhanapal AND R.Manavalan Antioxidant, coleous vettiveroides, DPPH method, Nitric oxide method, total antioxidant activity 353-357