10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) A spectrophotometric method has been developed for the determination of Cu(II) using Acetophenone 2',5'dihydroxy, semicarbazone lessThan sup greaterThan 1 lessThan /sup greaterThan as an extractive reagent .The reagent forms a colored complex which has been quantitatively extracted into n-Butanol at pH 5.6. The method obeys Beer's law over a range of 1 to 10 ppm. The molar absorptivity is 2564.1 L mol lessThan sup greaterThan -1 lessThan /sup greaterThan cm lessThan sup greaterThan -1 lessThan /sup greaterThan and Sandell's sensitivity is 0.02484 µg cm lessThan sup greaterThan -2 lessThan /sup greaterThan respectively. The proposed method is very sensitive and selective. This method has been successfully applied to synthetic and commercial samples. R. S. Lokhande, S. P. Janwadkar, Santosh Kulkarni And Sanjay Patil Copper, Spectrophotometric determination, n-Butanol, Acetophenone 2’, 5’-dihydroxy, semicarbazone derivative. 315-319