International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 15 Issue 3, July-September, Pages:16-22

Evaluation of Anti-Epileptic Properties of Ashwagandha and Chamomile by PTZ-Induced Mouse Model

Dr. P.S. Venkatesan, M. Eswarya and M. Madhavaselvi

Ayurvedic treatments are used in various types of neurological disorders. One of the common neurological disorders is Epilepsy. Withania somnifera and Matricaria recutita are well known for their medicinal properties, such as antimicrobial, antioxidant, anti-inflammatory, and antiepileptic properties. There are also studies examining the properties of Withania somnifera and Matricaria recutita. This study aims to prove the antiepileptic properties of Withania somnifera and Matricaria recutita extracts by testing them on epilepsy-induced Swizz albino mice. Five groups of male Swizz albino mice of 40±5g of weight, each consisting of 5 mice. The drugs were administered at a dose level of 100 mg/kg b.wt each for a week. The inducing method was used to induce epilepsy. Pentylenetetrazol (PTZ) is injected, which acts as a GABA-A receptor antagonist. It stimulates the nerves and causes seizures. The first group acted as a control, the second group as a reference control, with Diazepam, and the remaining groups were dosed with the herbal compound orally before PTZ injection (120 mg/kg b.wt). The evaluation of the latency period and the seizure period was done. The results were obtained and analyzed with ANOVA. Chamomile and Diazepam showed longer latency periods of 102±42.64 s and 103.5±20.50 s, respectively. In the seizure period, the combination group showed good results, having fewer jerks in the clonic period, less than 10 s of the tonic period, and less than 8 s recovery. This showed the anti-epilepsy activity of the herbal extracts of Withania somnifera and Matricaria recutita.

Keywords: Withania somnifera, Matricaria recutita, Epilepsy, Seizures, Mice, Pentylenetetrazol.
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  1. Jahanbani, R. et al. Anti-seizure effects of walnut peptides in mouse models of induced seizure: The involvement of GABA and nitric oxide pathways. Epilepsy Res176, 106727 (2021).
  2. Santhosh, N., Sinha, S. &Satishchandra, P. Epilepsy: Indian perspective. Ann Indian Acad Neurol17, S3–S11 (2014).
  3. Bora, E. S., Karaali, R., Akyol, P., Yurtsever, G. & Erbas, O. The effect of sulfasalazine in pentylenetetrazole- induced seizures in rats. Brazilian Journal of Medical and Biological Research = Revista Brasileira de pesquisasmedicas e biological / Sociedade Brasileira de Biofisica ... [et al.]54, (2021).
  4. Abou-Khalil, B. & Schmidt, Dieter. Chapter 42 - Antiepileptic drugs: advantages and disadvantages. In Handbook of Clinical Neurology (eds. Stefan, H. & Theodore, W. H.) vol. 108 723–739 (Elsevier, 2012).
  5. Abdul, W. Difficulties in Treatment and Management of Epilepsy and Challenges in New Drug Development. Pharmaceuticals3, (2010).
  6. Huang, R. et al.Pentylenetetrazole-induced inhibition of recombinant gamma-aminobutyric acid type A (GABA(A)) receptors: mechanism and site of action. J Pharmacol Exp Ther298, 986–995 (2001).
  7. Li, B. et al. The Anticonvulsant Effects of SR 57227 on Pentylenetetrazole-Induced Seizure in Mice. PLoS One9, e93158 (2014).
  8. Akünal Türel, C. &Yunuso?lu, O. Oleanolic acid suppresses pentylenetetrazole-induced seizure in vivo. Int J Environ Health Res33, 529–540 (2023).
  9. Abdul Rafique, D. Herb’s Used in Psychological Disorders. Inventi Rapid: Ethnopharmacology1, 1–10 (2010).
  10. Singh, G. & Kaur, P. ANTIPSCYHOTIC INDIAN HERBAL FORMULATIONS: AN OVERVIEW. Indian Research Journal of Pharmacy and Science04, 915–924 (2017).
  11. Sah, A. et al. A Comprehensive Study of Therapeutic Applications of Chamomile. Pharmaceuticals15, 1284 (2022).
  12. Kumar, R., Saifi, A. & Kumar, M. Ayurvedic Treatment of Panic Disorder. Research Journal of Pharmacology and Pharmacodynamics13, 2321–5836 (2021).
  13. Sayyar, Z., Yazdinezhad, A., Hassan, M. & Jafari Anarkooli, I. Protective Effect of Matricaria chamomilla Ethanolic Extract on Hippocampal Neuron Damage in Rats Exposed to Formaldehyde. Oxid Med Cell Longev2018, 6414317 (2018).
  14. DANTAS, J. B. de L. et al. Evaluation of the effect of Matricaria recutita monotherapy or in combination with photodynamic therapy on tissue repair in the dorsum of the tongue of rats*. Journal of Applied Oral Science31, (2023).
  15. Kazemi, M., Ghavipanjeh, G., Shahaboddin, M. E. &Banitaba-Bidgoli, S. M. The effect of hydro-alchoholicexteract of (Matricaria recutita L.)  on pentylenetetrazole-induced seizure and its relationship with nitric oxide in mice. KAUMS22, 346–354 (2018).
  16. Gaurav, N. et al. MORPHOLOGY OF WITHANIA SOMNIFERA (Distribution, Morphology, Phytosociology of Withaniasomnifera L. Dunal). 164–173 (2015).
  17. Shenoy, S., Chaskar, U., Sandhu, J. &Paadhi, M. Effects of eight-week ashwagandha supplementation on cardiorespiratory endurance in elite Indian cyclists. J Ayurveda Integr Med3, 209–214 (2012).
  18. Mishra, L. C., Singh, B. B. & Dagenais, S. Scientific basis for the therapeutic use of Withaniasomnifera (Ashwagandha): A Review. Altern Med Rev5, 334–346 (2000).
  19. Singh, N., Bhalla, M., de Jager, P. & Gilca, M. An Overview on Ashwagandha: A Rasayana (Rejuvenator) of Ayurveda. African journal of traditional, complementary, and alternative medicines?: AJTCAM / African Networks on Ethnomedicines8, 208–213 (2011).
  20. Gowda, G., Das, K., Bhosle, V., Einstein, J. & K, B. Evaluation of anticonvulsant activity of ethanolic leaves extract of Desmodiumtriflorum in mice. Revista Brasileira de Farmacognosia22, 649–656 (2012).
  21. C., M., Prasad, V. & I., S. Anticonvulsant effect of nifedipine, diazepam and in combination on pentylenetetrazol-induced experimental models of epilepsy on albino rats. Int J Basic Clin Pharmacol (2017) doi:10.18203/2319-2003.ijbcp20174634.\
  22. Johnson, Wilbur, I.Boyer., Amended Safety Assessment of Chamomilla recutita-Derived Ingredients as Used in Cosmetics. International Journal of Toxicology 37 (2018): 51S - 79S.
  23. Asadi-Shekaari, M., Eslami, A., Kalantaripour, T. &Joukar, S. Potential Mechanisms Involved in the Anticonvulsant Effect of Walnut Extract on Pentylenetetrazole-Induced Seizure. Med Princ Pract23, (2014).
  24. ergülerkeç, özlem& ARIHAN, O. Pentylenetetrazole Kindling Epilepsy Model. Epilepsi21, 6–12 (2015).
  25. Zolkowska, D. et al. Characterization of Seizures Induced by Acute and Repeated Exposure to Tetramethylenedisulfotetramine. J Pharmacol Exp Ther341, 435–446 (2012).
  26. Hammond, N. Tonic–Clonic Seizures?. in Reference Module in Biomedical Sciences (Elsevier, 2016). doi
  27. Stafstrom, C. & Carmant, L. Seizures and Epilepsy: An Overview for Neuroscientists. Cold Spring Harb Perspect Med5, a022426–a022426 (2015).
  28. Introduction. in The Causes of Epilepsy: Common and Uncommon Causes in Adults and Children (eds. Shorvon, S. D., Andermann, F. & Guerrini, R.) 1–42 (Cambridge University Press, Cambridge, 2011). doi:DOI: 10.1017/CBO9780511921001.003.
  29. Stafstrom, C. E. The pathophysiology of epileptic seizures: a primer for pediatricians. Pediatr Rev19, 342–351 (1998).
  30. Mody, I. & Pearce, R. A. Diversity of inhibitory neurotransmission through GABAA receptors. Trends Neurosci27, 569–575 (2004).
  31. Ramsdell, J. S. & Stafstrom, C. E. Rat kainic acid model provides unexpected insight into an emerging epilepsy syndrome in sea lions. Epilepsy Curr9, 142–143 (2009).
  32. Abdelbasset, W. K. et al. Treatment of pilocarpine-induced epileptic seizures in adult male mice. Braz J Biol84, e260091 (2022).
  33. Alharbi, K. S. Anticonvulsant effects of desvenlafaxine on modulating brain monoamine and oxidative stress in mice. Braz J Biol83, e246194 (2021).
  34. Umukoro, S. et al. Evaluation of the anticonvulsant and anxiolytic-like activities of aqueous leaf extract of Cymbopogon citratus in mice. J Basic Clin PhysiolPharmacol31, (2019).
  35. Iniaghe, L. O., Ighodaro, I., Magaji, M. G., Tabot, T. P. & Maduka, I. T. Neurobehavioural evaluation of Lophiraalata (Ochnaceae) stem bark extract in mice. J Basic Clin PhysiolPharmacol26, 523–529 (2015).
  36. Abdulsahib, W. K., Kathem, S. H., Al-Radeef, M. Y. & Jasim, L. S. Mentha piperita Oil Exerts an Antiepileptic Effect in Pilocarpine and Pentylenetetrazol-Induced Seizures in Mice. Vet Med Int2022, 4431317 (2022).
  37. Bhat, J. U. et al. Anticonvulsant activity of methanolic and aqueous extracts of Melissa parviflora in experimentally induced Swiss albino mice. EXCLI J11, 1–6 (2012).
  38. Rahmati B, Zaeri F, Heydari A. Proconvulsant effects of Nepeta menthoides hydro alcoholic extract in different seizure tests: behavioral and biochemical studies. Heliyon. 25;6(11):e05579 (2020). 
  39. Ya’u, J. et al. Anticonvulsant activity of aqueous fraction of Carissa edulis root bark. Pharm Biol53, 1329–1338 (2015).
  40. Tanna, I., Aghera, H., Bk, A. & Chandola, H. Protective role of Ashwagandharishta and flax seed oil against maximal electroshock induced seizures in albino rats. Ayu33, 114–118 (2012).
  41. Sulaiman, A. Study of anticonvulsant effect of ethyl acetate fraction of Matricaria recutita extract in mice. Int J Pharm Pharm SciVol 6, 224–227 (2014).
  42. Duan, J., Wang, J., Zhao, Q., Wu, D. & Liu, Y. Anti-convulsant Effects of Scutellarein in a PTZ Kindling Model in Mice. Pharmacogn Mag20, 347–356 (2023).
  43. Pithadia, A. et al. Reversal of experimentally induced seizure activity in mice by glibenclamide. Ann Neurosci20, 10–12 (2013).
  44. Amabeoku, G. J., Leng, M. J. & Syce, J. A. Antimicrobial and anticonvulsant activities of Viscum capense. J Ethnopharmacol61, 237–241 (1998).
  45. Fradley, R. L. et al. Differential contribution of GABAA receptor subtypes to the anticonvulsant efficacy of benzodiazepine site ligands. Journal of Psychopharmacology21, 384–391 (2007).
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