There is a growing interest in the chemical composition of plant-based medicines. Bioactive constituents have been isolated and studied for pharmacological activities. Plant-based drugs remain an important source of therapeutic agents because of their availability, relatively cheaper cost, and non-toxic nature compared to modern medicine. Cocculus hirsutus L., locally called Jaljamini, belongs to Menispermaceae and is a climbing scandent shrub with hairy sepals. The leaves of C. hirsutus L. were screened to identify and analyze the phytochemical constituents responsible for developing new drugs. The preliminary phytochemical analysis showed the presence of major phytoconstituents like alkaloids, phenolic compounds, flavonoids, etc. The studies on the bioactive components in the methanolic seed extract of C. hirsutus L. by GC-MS analysis showed the presence of four bioactive compounds such as 2-hexyldecanoic acid (220.19%), 2-Hydroxy–14–methyl– oxa – cyclotetradec – 6 – en – 2 – one (122.04%), 11-Hexadecyn –1–ol (622.56%), 6, 9 - octadecadienoic acid methyl ester (226.27%). The infrared spectrum analysis of methanolic and aqueous seed extracts of C. hirsutus L. showed the presence of alkyl halide, alcohol, aromatic, acid, and alkyne functional groups. The UV-VIS spectrum analysis results of methanolic and aqueous seed extract of C. hirsutus L. showed absorption at 311 nm (-0.403) and 310 (-0.110), respectively. The results obtained in our study suggest that, the leaf extract of Cocculus hirsutus L. can be a promising candidate for the development of phytomedicine in the treatment and prevention of various ailments.
World Health Organization. Principles of laboratory animal care. World Health Organization Chronicle. 1985; 39: 51-56.
Izzo AA and Ernst E. Interactions between herbal medicines and prescribed drugs. 2009; 69(13): 1777-1798.
Liu Y, Dao Z, Yang C, Liu Y and Long C. Medicinal plants used by Tibetans in Shangri-la Yunnan. China Journal of Ethnobiology and Ethnomedicine. 2009; 5(1): 15.
Kebriaee-zadeh, Overview of Iran's national drug policy. Iranian Journal of Pharmaceutical Research. 2003; 2: 1-2.
Talalay P and Talalay P. The importance of using scientific principles in developing medicinal agents from plants Academic Medicine. 2001; 76(3): 238-247.
Nostro A, Germano MP, D'angelo V, Marino A and Cannatelli MA. Extraction methods and bioautography for evaluating medicinal plant antimicrobial activity Letters in applied microbiology. 2000; 30(5): 379-384.
Ncube NS, Afolayan AJ and Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends African Journal of biotechnology. 2008; 7(12): 1797-1806.
Khalid A, Rehman U, Sethi A, Khilji S, Fatima U, Khan MI and Mahmood S. Antimicrobial analysis of Acacia modesta, Artemisia Absinthium, Cocculus hirsutus, and Saussurea lappa against Gram positive and Gram negative microorganisms. African Journal of Biotechnology. 2011; 10(22): 4574-4580.
Zohary D, Hopf M and Weiss E. Domestication of Plants in the Old World: The origin and spread of Domesticated Plants in Southwest Asia, Europe, and the Mediterranean Basin Oxford University Press on Demand.2012.
Tahan M and Bayram I. Effect of using Cocculus hirsutus and Petroselinum crispum in laying quail diets on egg yield, egg quality and hatchability Archiva zootechnica. 2011; 14(4): 39-44.
Mathur ML, Gaur J, Sharma R, and Haldiya KR. Antidiabetic properties of a spice plant Cocculus hirsutus. Journal of Endocrinology and Metabolism. 2011; 1(1): 1-8.
Javed S, Shahid AA, Haider MS, Umeera A, Ahmad R, and Mushtaq S. Nutritional, phytochemical potential and pharmacological evaluation of Cocculus hirsutus and Trachyspermum Ammi. Journal of Medicinal Plants Research. 2012; 6(5): 768-775.
Brindha P and Saraswathy A. Phytochemical comparison of Pentatropis, Oldenlandia, and Plumeria In Proc. Natl. A seminar on Recent Trends in Natural Products Chemistry was held on March 30-31 at Bharathidasan Univ., Tiruchirappalli, India. 1981.
Sania Feroz and Ghias Uddin. Phytochemical Analysis, Antimicrobial and Antioxidant Study of Cocculus hirsutus L. International Journal of Pharmacy and Chemistry. 2016; 2(2): 39-43.
Aisha Kamal and Iffat zareen Ahmad, Phytochemical studies of different phases of germination of Cocculus hirsutus L. - a medicinally important plant. Int J Pharm Sci. 2014; 6(4): 318-323.
Krishnaveni M and Saranya S. Phytoconstituent analysis of Cocculus hirsutus L. using analytical techniques Bull. Environ. Pharmacol. Life Sci. 2016; 5(3): 25-38.
Hadi MY, Mohammed GJ and Hameed IH. Analysis of bioactive chemical compounds of Cocculus hirsutus L. using gas chromatography-mass spectrometry. Journal of Pharmacognosy and Phytotherapy. 2015; 8(2): 8-24.
Singh N, Pandit V, Verma M and Mehta BK. GC-MS study of Cocculus hirsutus L. fatty oil Grasas y Aceites. 2002; 53(2): 173-174.
Nivetha K and Prasanna G. GC-MS and FT-IR analysis of Cocculus hirsutus L. Int. J. Adv. Res. Biol. Sci. 2016; 3(6): 45-54.
Silverstein RM, Bassler GC and Morrill TC. Spectroscopic identification of organic compounds. John Wiley and Sons. 1981, New York.
Dhivya KK. Screening of phytoconstituents, UV-VIS Spectrum and FT-IR analysis of Cocculus hirsutus L. International Journal of Herbal Medicine. 2017; 5(6): 40-44
Nithyadevi J and Sivakumar R. Phytochemical screening and GC–MS, FT-IR analysis of methanolic extract leaves of Cocculus hirsutus L. Sw. Int. J. Res. Studies in Biosci. 2015; 3(9): 61-66.
Rani N, Sharma S and Sharma, M. Phytochemical Analysis of Cocculus hirsutus L. by FT-IR and UV-VIS Spectrophotometer. Indian Journal of Science and Technology. 2016; 9(31).
Antony TS, Peter MJ, and Raj JY. Phytochemical Analysis of Cocculus hirsutus L. using UV-VIS, FT-IR, and GC-MS. Research Journal of Chemical Sciences. 2013; 3(11): 14-23.
Janakiraman M and Jeyaprakash K. Evaluation of Phytochemical Compounds in Leaf Extract of Cocculus hirsutus L. using TLC, UV-VIS and FT-IR analysis. International Journal of Health Sciences and Research (IJHSR). 2015; 5(8): 289-295.
Brahman P and Sunita K. Phytochemical investigation and In-vitro antimalarial Activity of Acalypha indica (L.) and Cocculus hirsutus (L.) from Prakasam District, Andhra Pradesh, India, Biomed Pharmacol Journal. 2018; 11(4): 1-4.
Nayyar K, Arora U, Palla S, Prasad M, Saravan A, Madan S, Sood R, Khanna N, Potdar A and Shukla R. Use of Cocculus hirsutus Extract for Treating Dengue. PCT International Application. 2020; 2(20): 115-123.