International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 9 Issue 2
2018 (April-June)
Effect of probiotic encapsulated Artemia Nauplii on the digestive enzyme activity in post larvae of Penaeus Monodon
Though Global shrimp production and trading had significantly increased in the past 20 years, loss due to disease remained as a major problem. Vibriosis caused by lessThan i greaterThan Vibrio harveyi, V.anguillarium V.alginolyticus lessThan /i greaterThan is one of the major concern in shellfish aquaculture. Administration of probiotics is an effective alternative to the use of chemicals and antibiotics for the prevention of pathogens. Probiotics are the live microorganisms which are known for their ability to improve growth, water quality and immune responses of cultured species. lessThan i greaterThan Bacillus, Lactobacillus sps lessThan /i greaterThan are commonly used as probiotics in shrimp aquaculture as supplementation along with feed or directly inoculated into rearing systems. The peculiar feeding characteristics of brine shrimp, lessThan i greaterThan Artemiafranciscana lessThan /i greaterThan enabled its use for delivering vaccines. Hence, the present study is carried out to understand the influence of bioencapsulated lessThan i greaterThan Bacillus, Lactobacillus Artemia lessThan /i greaterThan nauplii in the enhancement of digestive enzymes in lessThan i greaterThan Peneaus monodon lessThan /i greaterThan . Amplification of lessThan i greaterThan B.subtilis lessThan /i greaterThan by 83%, 99.5% and 107% and lessThan i greaterThan L.sporogenes lessThan /i greaterThan by 52%, 64.5% and 86% administered to 2 lessThan sup greaterThan nd lessThan /sup greaterThan instarartemianauplii secured following 3, 5 and 8hrs of feeding respectively indicating this potential use as bio encapsulating agent. Increase in lessThan i greaterThan B.subtilis lessThan /i greaterThan to 392 and 432 CFU/ml and lessThan i greaterThan L.sporogenes lessThan /i greaterThan to 300 and 356CFU/after Day1 and Day 2 respectively further ensured their colonization in the host. Feeding of encapsulated lessThan i greaterThan B.subtilis lessThan /i greaterThan up to 60 days resulted in 61, 46 and 76% increase in the activity of amylase, protease and lipase respectively while encapsulated lessThan i greaterThan L.sporogenes lessThan /i greaterThan diet resulted in 35,17and 63% increase in the same. Hence, this study clearly showed the ensured delivery of gut probiotics through bioencapsulation to obtain the anticipated benefit.
S. JYOTHSNA, D. SAI VINATHI, V. KALARANI
Bioencapsulation, Probiotics, digestive enzymes, Peneaus monodon
266-272