International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 3
2017 (July - September)
Deciphering the competence of microbial isolate in Synthetic consortium for dairy wastewater treatment (Funded Work)
The present study was designed in two phases. In phase I,fourteen bacteria and six fungi were isolated from dairy effluent and their individual COD (Chemical Oxygen Demand) and BOD (Biological Oxygen Demand) removal efficiency was evaluated. Four microbial isolates E3, E5, E11 and F5 exhibiting more than 50% COD and BOD reduction were selected for formulation of synthetic consortium. On the basis of 16S and 5.8S rRNA sequencing these were identified as E3 ( lessThan i greaterThan Serratia marcescens lessThan /i greaterThan ), E5 ( lessThan i greaterThan Stenotrophomonas maltophilia lessThan /i greaterThan ), E11 ( lessThan i greaterThan Brachybacetrium muris lessThan /i greaterThan ) and F5 ( lessThan i greaterThan Cunninghamella echinulata lessThan /i greaterThan ). In phase II, four synthetic consortia were designed by incorporating the isolates in different proportion and combinations to treat three forms of dairy effluents .Consortium set D (5% lessThan i greaterThan Serratiamarcescens lessThan /i greaterThan + 5% lessThan i greaterThan Cunninghamella echinulata lessThan /i greaterThan + 3% lessThan i greaterThan Stenotrophomonas maltophilia lessThan /i greaterThan + 3% lessThan i greaterThan Brachybacetrium muris lessThan /i greaterThan )exhibited maximum level of removal of COD (89% ) and BOD (78%). This communication revealedthat exploitation of such efficient consortium (set D) can overcome the inefficiencies pre-existing with the biological wastewater treatment plants by acting as prospective candidate for bio-augmenting the native microflora.
ANUPAMA BHARDWAJ, JAGTAR SINGH,
AND SONIA CHAMAN
Dairy effluent, biological treatment, bacterial isolates, fungal isolates, consortium, molecular
characterization
1109-1114