International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 3
2017 (July - September)
Insilico screening of biocomponents in cympobogan citratus and allium sativum against microsporum sp
Effective and nontoxic antifungal agents are required for the treatment of dermatophytosis. Plants and their extracts have been used as medicines against superficial infections. In our present study, from the Leaves of lessThan i greaterThan Cymbopogon citratus lessThan /i greaterThan (lemon grass) and pulp of lessThan i greaterThan Alllium sativum lessThan /i greaterThan (garlic) extracts were prepared by using different solvent i.e., Ethanol, Ethyl acetate, Chloroform and Aqueous extract using soxhlet apparatus and to investigate the antifungal activity. Water Samples were collected from sewage, swimming pools and lakes in Chennai, Tamilnadu. lessThan i greaterThan Microsporum gypseum lessThan /i greaterThan and lessThan i greaterThan Microsporum canis lessThan /i greaterThan were isolated and identified from the samples. Phytochemicals analysis with the Leaves of lessThan i greaterThan Cymbopogon citratus lessThan /i greaterThan (lemon grass) and pulp of lessThan i greaterThan Alllium sativum lessThan /i greaterThan (garlic) extracts were studied using different solvent i.e., Ethanol, Ethyl acetate, Chloroform and Aqueous extract MIC and MFC were carried out with the isolates, lessThan i greaterThan Cymbopogon citratus lessThan /i greaterThan extract with Ethyl acetate showed higher antidermatophytic activity, by inhibiting the lessThan i greaterThan Microsporum canis lessThan /i greaterThan at the concentration of 62.5(µg/ml), and Ethanolic extracts the concentration of 125(µg/ml) and aqueous extract in the concentration of 500(µg/ml). lessThan i greaterThan Alllium sativum lessThan /i greaterThan extract lessThan i greaterThan with lessThan /i greaterThan Ethyl acetate showed higher antidermatophytic activity,by inhibiting the lessThan i greaterThan Microsporum gypseum lessThan /i greaterThan at the concentration of 62.5(µg/ml), and chloroform extract in the concentration of 250(ug/ml) and aqueous extract in the concentration of 1000(µg/ml). Gas Chromatography Mass Spectroscopy was done to identify the biochemical peak components and the activities present in the lessThan i greaterThan Cymbopogon citratus lessThan /i greaterThan and lessThan i greaterThan Alllium sativum lessThan /i greaterThan extracts. Seven compounds were identified with their following retention time. Citral (18.83), dimethyl sulfide (19.58) followed by nonadeconic acid (20.87), Brassidic acid (21.87), Behenic acid (22.82), hexodeconic acid (17.17) and Flavone (14.07). of which Flavone, citral and dimethyl sulphide acts as the ligands to bind with the specific proteins present in the organism and to have inhibitory activity. Molecular docking was done for the LAP1 protein and MEP1 proteins from lessThan i greaterThan Microsporum sp. lessThan /i greaterThan Citral compound found to have greater inhibitory property against the pathogenic dermatophytes lessThan i greaterThan . lessThan /i greaterThan Ethyl acetate extracts of lessThan i greaterThan Cymbopogon citratus lessThan /i greaterThan and lessThan i greaterThan Alllium sativum lessThan /i greaterThan have medicinal property to treat infectious pathogenic fungi.
G. KRISHNAVENI AND DR. E. M. RAJESH
Dermatophytes, Medicinal plants, phytochemical analysis, Microsporum gypseum, Microsporum canis, GC-MS, Molecular docking.
691-700