International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 3
2017 (July - September)
Docking and antidermatophytic studies of azadirachta indica With aloe barbadensis against trichophyton species
The study was to assess the antidermatophytic activity of the ethanol, ethyl acetate and aqueous extract of Neem ( lessThan i greaterThan Azadirachta indica lessThan /i greaterThan ) and Aloe vera ( lessThan i greaterThan Aloe barbadensis lessThan /i greaterThan lessThan i greaterThan ) lessThan /i greaterThan against dermatophytes isolated from different soil. Dermatophytes were identified, Out of 156 samples, 42 isolates of lessThan i greaterThan Trichophyton mentagrophytes lessThan /i greaterThan , 37 isolates of lessThan i greaterThan Trichophyton rubrum lessThan /i greaterThan , 27 isolates of lessThan i greaterThan Trichophyton terrestre lessThan /i greaterThan , 21 isolates of lessThan i greaterThan Trichophyton tonsurans lessThan /i greaterThan , and 18 isolates of lessThan i greaterThan Trichophyton verrucosum lessThan /i greaterThan were isolated and identified. We selected two most common pathogen among the lessThan i greaterThan Trichophyton lessThan /i greaterThan species, i.e., lessThan i greaterThan Trichophyton mentagrophytes lessThan /i greaterThan and lessThan i greaterThan Trichophyton rubrum lessThan /i greaterThan to find the antidermatophytic activity of Plant extracts. The minimum inhibitory concentration (MIC) by broth dilution method and minimum fungicidal concentration (MFC) with the leaf extracts of the plants lessThan i greaterThan Azadirachta indica lessThan /i greaterThan and lessThan i greaterThan Aloe barbadensis lessThan /i greaterThan against the isolated dermatophytes was determined. Among the lessThan i greaterThan Trichophyton lessThan /i greaterThan species, The MIC of the combination of ethyl acetate extract of lessThan i greaterThan Azadirachta indica lessThan /i greaterThan with lessThan i greaterThan Aloe barbadensis lessThan /i greaterThan shows the least MIC valve of 62.5µg/ml, to inhibit lessThan i greaterThan T. mentagrophytes lessThan /i greaterThan and 125µg/ml against the growth of lessThan i greaterThan T. rubrum lessThan /i greaterThan . Controls were maintained. The GC-MS chromatogram analysis of combined lessThan i greaterThan Azadirachta indica lessThan /i greaterThan with lessThan i greaterThan Aloe barbadensis lessThan b greaterThan lessThan /b greaterThan lessThan /i greaterThan showed the presence of eight components based on their molecular weight and retention time. Phytol derivative shows the highest peak at a retention time of 18.83. lessThan i greaterThan In silico lessThan /i greaterThan molecular docking was carried out to find the molecular interaction analysis of four compounds (phytol, heptadecanoic acid, isopropyl stearate and docosanoic acid) against glucan1,3- beta glucosidase and subtilisin like protease 3 using Autodock software. These two proteins selected for our study are which was an extracellular protease secreted due to the dermatophytic infection. Among the four ligands, phytol shows good interactions with least energy against drug target hence it may act as potent inhibitor against lessThan i greaterThan T. mentagrophytes and T. rubrum. lessThan /i greaterThan The results prove that combination of lessThan i greaterThan Azadirachta indica lessThan /i greaterThan with lessThan i greaterThan Aloe barbadensis lessThan /i greaterThan can be used to develop a novel drug formulation against tinea infection. The lessThan i greaterThan insilico lessThan /i greaterThan studies can be further analysed for innovative and informative treatment methods.
C. BANU REKHA, DR.D.LATHA AND DR. S.VIJAYALAKSHMI
Soil, Trichophyton, Azadirachta indica and Aloe barbadensis, Molecular docking.
592-600