International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 7 Issue 2
2016 (April - June)
PHYTOCHEMICAL COMPOSITION, IN VITRO ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF SEEDS OF CONVOLVULUS ARVENSIS LINN.
In vitro antioxidant and cytotoxic activities of methanol extract of the seeds of lessThan i greaterThan Convolvulus arvensis lessThan /i greaterThan Linn. and its successive fractions; chloroform, ethyl acetate and lessThan i greaterThan n lessThan /i greaterThan -butanol were evaluated. Total phenolics and flavonoid contents of methanol extract and ethyl acetate fraction were estimated. The highest antioxidant activity was achieved in ethyl acetate fraction (120.3±0.55 mg GAE) followed by methanol extract (84.8±0.62 mg GAE). The ethyl acetate fraction showed the strongest DPPH and nitric oxide free radical scavenging activities (IC lessThan sub greaterThan 50 lessThan /sub greaterThan 28.16±0.45 and 51.92±1.45 for DPPH and nitric acid, respectively). Ethyl acetate fraction showed the highest cytotoxic effect against Hela cell line (IC lessThan sub greaterThan 50 lessThan /sub greaterThan 17.33±0.58). From ethyl acetate fraction seven compounds were isolated and identified; gallic acid (1), ferulic acid (2), chlorogenic acid (3), quercetin (4), apigenin-7- lessThan i greaterThan O lessThan /i greaterThan -glucoside (5), quercetin-7- lessThan i greaterThan O lessThan /i greaterThan -rhamnoside (6) and quercetin-3- lessThan i greaterThan O lessThan /i greaterThan -rutinoside (7) using different spectroscopic techniques. Therefore, methanolic extract and the ethyl acetate fraction of the seeds of lessThan i greaterThan Convolvulus arvensis lessThan /i greaterThan are recommended to be used as natural antioxidant and cytotoxic agents.
TAGHREED A. IBRAHIM, ATEF A. EL-HELA, NEVEIN M. ABD ELHADY AND NABIL M. ABO-ELFETOH
Antioxidant, Convolvulus arvensis, Cytotoxic, seeds, phenolic acids, flavonoid
107-116