International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 7 Issue 1
2016 (January - March)
PRODUCTION AND OPTIMIZATION OF L-GLUTAMINASE FROM MANGROVE ISOLATE AND COMPARATIVE MUTATIONAL STUDIES
Actinomycetes, isolated from marine and mangrove sediment samples, were evaluated for the production of L-glutaminase. All the isolates were screened for L-glutaminase production and the one exhibiting maximum glutaminolytic activity was selected as potential L-glutaminase producer and used for mutational studies. Both wild and mutant strains were used for L-glutaminase production by solid state fermentation using different agro- industrial by-products such as green gram, black gram, coconut oil cake, sesame oil cake, rice bran and wheat bran. Sesame oil cake was the best substrate for induction of L-glutaminase (153.066 U/gds by wild strain and 158.53 U/gds by mutant strain). Culture conditions like initial moisture content, inoculum volume, temperature and pH of the culture medium were optimized using a 2 lessThan sup greaterThan 4 lessThan /sup greaterThan full factorial CCD (Central Composite Design) and a second order polynomial model equation was obtained. The predicted optimum levels were as follows: temperature 36.21 lessThan sup greaterThan 0 lessThan /sup greaterThan C, inoculum volume 1.87 ml, initial moisture content 69.47 % (v/w) and pH 8.2. Under these optimum conditions, the experimental yield of L-glutaminase was 203.86 U/gds, which was in close agreement with the value predicted by the model, 206.57 U/gds. In the present study the value of the regression coefficient R lessThan sup greaterThan 2 lessThan /sup greaterThan = 0.9894 which indicates that 98.94% of the variability in the response could be explained by the model.
D. DIVYA TEJA , V. SRIDEVI, N. HARSHA AND A. SWATHI
Actinomycete, L-glutaminase, mangrove, mutant, solid state fermentation, wild, central composite design
48-60