International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 1 Issue 2
2010 (April - June)
Effect of Trace Elements on Biosorption of HG2+ By HG2+Tolerant Saccharomyces Cerevisiae A100
Use of microorganisms for removing mercury (Hg++) is an effective technology for the treatment of industrial wastewaters and can become an effective tool for the remediation of man-impacted coastal ecosystems with this metal. In our laboratory a high Hg++ resistant Saccharomyces cerevisiaeA100 is already developed to facilitate Hg++ biosorption even from Hg++ rich solution. Inorganic anions and cations are essential for the growth and metabolism of Saccharomyces cerevisiaeA100 and also play significant role on biosorption process. Experiments revealed 0.15% K2HPO4, 0.06% KCl , 0.06% MgSO4,7H2O as the optimized inorganic ingredients of the nutrient medium (Glucose 5.0%, Urea 0.25%). Supplementation of nutrient broth with 1µg/ml FeSO4,7H2O, 5µg/ml MnSO4,H2O and 10µg/ml Na2MoO4,2H2O exhibit marked positive effect on biosorption of Hg++. Optimization of the concentration of inorganic nutrients enhances biosorption efficiency of Saccharomyces cerevisiaeA100 from 90% to 96.6% (developed significance level p lessThan 0.001) . Addition of CuSO4,5H2O; ZnSO4,7H2O; CoCl2,6H2O; NiSO4,7H2O; NH4VO3 to the biosorption medium was found to play inhibitory role on the growth and biosorption of Hg++ by Saccharomyces cerevisiaeA100.
Khushi Mukherjee and Ajit Kumar Banik
Biosorption, Mercury, Saccharomyces cerevisiae, Trace element
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