International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
TRIBUTYRIN-ESTERASE BIOSYNTHESIS IN Geobacillus sp. AGP-04 A STUDY OF REGULATORY MECHANISM.
Microbial esterases have a promising biotechnological potential to be used in various industrial purposes from the food industry to pharmaceutical industry. To accomplish appropriate function and higher quantity of the biocatalysts there is a need to govern the specific control of the enzyme synthesis. This article reports the regulation of a trybutyrin-esterase synthesis in a thermophilic bacterium, lessThan i greaterThan Geobacillus sp. lessThan /i greaterThan AGP-04. Tributyrin alone itself induced esterase synthesis. Other triglycerides, sugar-alcohol and other carbon sources exhibited little or no enzyme induction. Lower concentration (0.01–0.15% v/v) of the enzyme catalyzed end products, i.e. glycerol and butyric acid in the presence of tributyrin induced enzyme synthesis though higher concentration ( greaterThan 0.15%) was inhibitory. The addition of superfluous energy rich phosphonucleotides did not enhance the enzyme synthesis. Addition of glucose (about 0.2%) to the media supplemented with tributyrin repressed tributyrin-esterase biosynthesis. cAMP alleviated the enzyme synthesis in the glucose mediated repressed cell. Potent ionophores like 2-4-dinitrophenol and Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) repressed enzyme synthesis by inhibiting ATP synthesis in the cell. The study revealed that tributyrin-esterase synthesis to be an energy dependent process and it is under the control of catabolic repression.
AMIT GHATI AND GOUTAM PAUL
Tributyrin; Esterase; Geobacillus sp.; Enzyme regulation;Catabolic repression
454-465