International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
EFFECT OF EXOGENOUS INDOLE-3-BUTYRIC ACID AND INDOLE-3-ACETIC ACID ON BIOMASS AND LEGENDARY WITHANOLIDES FROM IN VITRO ROOT CULTURES OF WITHANIA SOMNIFERA – JAWAHAR 20 CULTIVAR
"Withanolides", the legendary bioactive metabolite present in leaves and roots of lessThan i greaterThan Withania somnifera, lessThan /i greaterThan is well known for its therapeutic activities since ancient times lessThan i greaterThan . lessThan /i greaterThan Several efforts have been made to produce these metabolites under lessThan i greaterThan in vitro lessThan /i greaterThan condition. lessThan i greaterThan lessThan /i greaterThan In the present study, lessThan i greaterThan lessThan /i greaterThan we have tested the effects of exogenous supplementation of IBA and IAA in lessThan i greaterThan W. somnifera lessThan /i greaterThan lessThan i greaterThan in vitro lessThan /i greaterThan root cultures with the aim of optimizing the production of biomass and major withanolides. Among the two auxins, IBA is an effective inducer of lateral root formation thereby increasing the biomass. Accumulation of withanolide A and the biomass increases as the concentration of IBA increased to 1mg/L (2576±0.37 µg/g DW and 12.89±0.25 g/dL respectively). IAA at lower concentration favours relatively high accumulation of withanolide A (1147±0.77 µg/g DW) and at higher concentration of 1mg/L helps in higher accumulation of withaferin A (624±0.87 µg/g DW).
PANKAJAVALLI. T, KALAISELVI. R, PRADEEPA.D AND KALAISELVI SENTHIL
Withania somnifera, in vitro adventitious root, IBA, IAA, withanolides, HPTLC
971-979