International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 3
2014 (July- September)
PREVALENCE AND SUSCEPTIBILITY PROFILES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN THE UNIVERSITY OF GHANA HOSPITAL, LEGON, ACCRA, GHANA
Methicillin-resistant lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan (MRSA) is one of the important causal agents of nosocomial infections worldwide. The prevalence and susceptibility profiles of MRSA in the University of Ghana Hospital, Legon, Accra were investigated from 65 swab samples. Samples were inoculated onto blood agar plates and incubated at 37°C aerobically for 24 h. After overnight incubation, isolates were tested biochemically and a total of 11 (16.9%) isolates of lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan were identified from the swab samples. The lessThan i greaterThan S. aureus lessThan /i greaterThan isolates were later confirmed as MRSA by growth on Oxacillin-Resistant Screening Agar Base (ORSAB) and detection of the lessThan i greaterThan mec lessThan /i greaterThan A gene using polymerase chain reaction (PCR) technique. Kirby-Baeur disc diffusion method was also used to determine the susceptibility of the isolates. There was only 1 (9.1%) MRSA isolate detected from the samples. The MRSA isolate was susceptible to co-trimoxazole, tetracycline, and gentamicin but resistant to ampicillin, cephalexin, cefotaxime, ciprofloxacin, prulifloxacin, ofloxacin, cloxacillin, roxithromycin, lincomycin, and cefoxitin antibiotics used in this investigation. Despite the low prevalence rate in this study, there is still the need for strong continuous surveillance programs to monitor MRSA and its antibiotic profiles.
HOSPITAL, LEGON, ACCRA, GHANA GEORGE A. PESEWU, ROGER DOGBE, RICHARD H. ASMAH, MICHAEL A. OLU-TAIWO AND DAVID N. ADJEI
Staphylococci, Methicillin-sensitive Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Polymerase Chain Reaction
185-193