International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 1
2014 (January - March)
OPTIMIZATION OF CULTURE CONDITIONS AND MEDIA COMPONENTS FOR LACCASE PRODUCTION USING WOOD ROTTING FUNGAL ISOLATE
The present work focuses on optimizing the culture conditions and media components for enhanced production of laccases using wood rotting fungal isolate. Parameters such as shaking or static conditions, agitation rate, pH and time for higher enzyme production were monitored. Medium for optimum laccase production was analysed using one factor at a time method and Taguchi orthogonal array method. One factor at a time method indicated that maltose, meat extract and ammonium sulphate were optimal carbon and nitrogen sources. 1% maltose, 0.750% meat extract, 0.124% ammonium sulphate (NH lessThan sub greaterThan 4 lessThan /sub greaterThan ) lessThan sub greaterThan 2 lessThan /sub greaterThan SO lessThan sub greaterThan 4 lessThan /sub greaterThan and 0.1% potassium phosphate (KH lessThan sub greaterThan 2 lessThan /sub greaterThan PO lessThan sub greaterThan 4 lessThan /sub greaterThan ) are found to be optimum concentrations for laccase production by Taguchi method. Under these optimized conditions laccase production increased by 49%. Enzyme production using various inducers showed that 2,6-xylidine was the most effective inducer.
PRIYANKA PUSHKARAJ VARTAK AND DR. DEBJANI DASGUPTA
laccase, wheat bran, 2,6-xylidine, basidiomycetes
801-809