International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 3
2013 (July - September)
GREEN SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF ACALYPHA INDICA AND ITS ANTIMICROBIAL ACTIVITY
In the present study, bio synthesis of silver Nanoparticles using aqueous extract of lessThan i greaterThan Acalypha indica lessThan /i greaterThan and its antimicrobial activity against different micro organisms were investigated. About 10 ml of aqueous extract of lessThan i greaterThan A. indica lessThan /i greaterThan added with 90 ml of AgNo lessThan sub greaterThan 3 lessThan /sub greaterThan (1mM) solution, the resulting mixture was incubated at 37 lessThan sup greaterThan â—¦ lessThan /sup greaterThan C under static condition. The development of yellowish brown color indicated the formation of Ag-Np's. The Ag-Np's monitored with the help of UV-visible spectrophotometer at the wavelength of 200– 800 nm. The observed absorbance peak at 400 nm indicated the formation of Ag -Np's. The particle size of Ag-Np's was determined by using particle analyzer and the results showed that average size range was found to be 0.516 nm.TEM technique was employed to visualize the size and shape of Ag-Np's. The antibacterial activity of lessThan i greaterThan A. indica lessThan /i greaterThan Ag-Np's was evaluated against both Gram positive and Gram negative pathogenic microorganism by disc diffusion method. The diameter of inhibition zone of lessThan i greaterThan A. indica lessThan /i greaterThan Ag-Np's was analyzed at different concentrations ranging from 100 to 300µg/ml. The maximum zone of inhibition was observed with lessThan i greaterThan P.aeruginosa lessThan /i greaterThan (16 mm), followed by lessThan i greaterThan E.coli lessThan /i greaterThan (14 mm), lessThan i greaterThan B.subtilis lessThan /i greaterThan ((13 mm) and lessThan i greaterThan S.aureus lessThan /i greaterThan (13 mm) when compared with standard drug Amikacin. The antifungal activity of lessThan i greaterThan A. indica lessThan /i greaterThan Ag-Np's at 300 µg/ml concentration was found to be 23mm and12 mm for lessThan i greaterThan C. albicans lessThan /i greaterThan & lessThan i greaterThan A. niger lessThan /i greaterThan respectively when compared with the standard antifungal drug ketokonazole. It is observed from the results that biologically synthesized Ag-Np's from lessThan i greaterThan A. indica lessThan /i greaterThan aqueous extract showed effective antimicrobial and antifungal activity against selected microorganisms which are comparable with standard.
D. KUMARASAMYRAJA AND N. S. JEGANATHAN
Ag-Np’s, Green synthesis, UV-Vis spectrometry, TEM, Antibacterial activity
469-476