International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 3 Issue 4
2012(October - December)
VOLATILE CONSTITUENTS AND ANTIOXIDANT PROPERTY OF ESSENTIAL OIL FROM PLECTRANTHUS AMBOINICUS (LOUR)
To investigate the lessThan i greaterThan in vivo lessThan /i greaterThan and lessThan i greaterThan in vitro lessThan /i greaterThan antioxidant activities of lessThan i greaterThan P. amboinicus lessThan /i greaterThan essential oil having important volatile constituents such a Carvocrol, Thymol, Cis/Trans - Caryophyllene, and P-Cymene. Methods: For lessThan i greaterThan in vitro lessThan /i greaterThan studies different concentration of essential oil ranging from 5 - 100µg/ml was used and the assay involves the DPPH free radical scavenging activity, Hydroxyl radical activity and reducing power activity. The lessThan i greaterThan in vivo lessThan /i greaterThan study on antioxidant activity, was carried out in a lung cancer mice model induced by injecting 100µl of B lessThan sub greaterThan 16 lessThan /sub greaterThan F- lessThan sub greaterThan 10 lessThan /sub greaterThan melanoma metastatic cell line suspension of about 1× 10 lessThan sup greaterThan 6 lessThan /sup greaterThan cells/ml in PBS into the tail-vein of C57BL/6 mice. The essential oil was given in the dose of 50µg/ml/dose for 21 days to each animal. The lessThan i greaterThan in vivo lessThan /i greaterThan study involves the assay of the level of Catalase, Superoxide Dismutase, Glutathione Peroxidase, Lipid peroxidation, Nitric oxide and reduced glutathione. Finally, Lung was dissected out for histopathology study to confirm the lung metastasis. Results: The essential oil exhibited significant inhibition in DPPH free radical and hydroxyl radical formation. Similarly, it has got a significant reducing power towards such reactive molecules. In animal studies, the level of antioxidant enzyme activity were found to be normalized in the essential oil treated group for Catalase, Superoxide dismutase, glutathione peroxidase and Lipid peroxidation. Whereas, the toxic molecule nitric oxide level has been reduced upon essential oil treatment. The non-enzymatic antioxidant reduced glutathione was found to be increased, in the essential oil of lessThan i greaterThan P. amboinicus lessThan /i greaterThan treated mice. The volatile compounds identified in GC – MS analysis in the essential oil were Carvocrol, Thymol, Cis – Caryophyllene, Trans – Caryophyllene, p- cymene as the major compounds with 96% comparison with the wilily and NBS library. Conclusion: The result obtained for both the lessThan i greaterThan in vitro lessThan /i greaterThan and lessThan i greaterThan in vivo lessThan /i greaterThan study shows that the essential oil of lessThan i greaterThan P. amboinicus lessThan /i greaterThan has a significant antioxidant activity due to the presence of important bioactive compounds and hence, it can be subjected for pharmaceutical drug formulations.
A.MANJAMALAI AND DR. V.M. BERLIN GRACE
Antioxidants, Essential oil, Free radicals, Plectranthus amboinicus (Lour), Volatile compounds.
445-458